LANDA, Přemysl, MARŠÍK, Petr, HAVLÍK, J., KLOUČEK, P., VANĚK, Tomáš, KOKOŠKA, L. Evaluation of Antimicrobial and Anti-Inflammatory Activities of Seed Extracts from Six Nigella Species. Journal of Medicinal Food. 2009, 12(2), 408-415. ISSN 1096-620X. E-ISSN 1557-7600

Seed extracts from six species of the genus Nigella (Family Ranunculaceae)-Nigella arvensis, Nigella damascena, Nigella hispanica, Nigella nigellastrum, Nigella orientalis, and Nigella sativa-obtained by successive extraction with n-hexane, chloroform, and methanol, were tested for their antimicrobial activity against 10 strains of pathogenic bacteria and yeast using the microdilution method as well as for anti-inflammatory properties by in vitro cyclooxygenase (COX)-1 and COX-2 assay. Chemical characterization of active extracts was carried out including free and fixed fatty acid analysis. Comparison of antimicrobial activity showed that N. arvensis chloroform extract was the most potent among all species tested, inhibiting Gram-positive bacterial and yeast strains with minimum inhibitory concentration (MIC) values ranging from 0.25 to 1 mg/mL.