DOLEŽELOVÁ, Marie, VALÁRIK, Miroslav, BARTOŠ, Jan, VRÁNA, Jan, ČIHALÍKOVÁ, Jarmila, MOLNÁR-LÁNG, M., DOLEŽEL, Jaroslav. Analysis and sorting of rye (Secale cereale L.) chromosomes using flow cytometry. Genome. 2003, 46(-), 893-905. ISSN 0831-2796. E-ISSN 1480-3321.

Procedures for chromosome analysis and sorting using flow cytometry were developed for rye (Secale cereale L.). Chromosome 1R could be discriminated on a flow caryotype. Remaining rye chromosomes (2R-7R) could be discriminated and sorted from individual wheat-rye addition lines. Flow sorted chromosomes were identified by fluorescence in situ hybridization. PCR with chromosome-specific primers confirmed the identity of sorted fractions and indicated suitability of sorted chromosomes for physical mapping. The possibility to sort large numbers of chromosomes opens a way for the construction of large-insert chromosome-specific DNA libraries in rye.