PLCHOVÁ, Helena, MORAVEC, Tomáš, DĚDIČ, P., ČEŘOVSKÁ, Noemi. Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production. Journal of Phytopathology - Phytopathologische Zeitschrift. 2011, 159(2), 130-132. ISSN 0931-1785. E-ISSN 1439-0434

Vector pMPM-A4 Omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp(43-616) and RdRp(304-537) were chosen for expression. A high level of CP and RdRp(304-537) was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.