ŠAFÁŘ, Jan, ŠIMKOVÁ, Hana, DOLEŽEL, Jaroslav. Construction of BAC libraries from flow-sorted chromosomes. In: KIANIAN, S.F., KIANIAN, P.M.A., eds. Plant Cytogenetics. 1429. New York: Humana Press, 2016, S. 135-149. Methods and Protocols. ISBN 978-1-4939-3620-5

Cloned DNA libraries in bacterial artificial chromosome (BAC) are the most widely used form of large-insert DNA libraries. BAC libraries are typically represented by ordered clones derived from genomic DNA of a particular organism. In the case of large eukaryotic genomes, whole-genome libraries consist of a hundred thousand to a million clones, which make their handling and screening a daunting task. The labor and cost of working with whole-genome libraries can be greatly reduced by constructing a library derived from a smaller part of the genome. Here we describe construction of BAC libraries from mitotic chromosomes purified by flow cytometric sorting. Chromosome-specific BAC libraries facilitate positional gene cloning, physical mapping, and sequencing in complex plant genomes.