Research on viruses and viral plant diseases
- Investigation of the molecular properties of plant viruses using immunochemical and molecular biological methods
- Mapping of plant virus genomes, virus isolates and phylogenetic analysis
- Preparation of polyclonal antibodies against recombinant structural and non-structural viral proteins to study the virus life cycle
- Detection of viruses using antibodies and/or methods based on DNA/RNA detection (LAMP, RT-LAMP, PCR, RT-PCR, qPCR)
Biotechnological utilisation of plant viruses
- Design of plant viral vectors based on different RNA and DNA viruses
- Adaptation of plant viral vectors to the GoldenBraid cloning system
- Transient expression of foreign proteins in plants
- Presentation of peptides/epitopes on the surface of virus particles (electron microscopy)
Metabolic engineering of plant cells
- Development of a platform for the expression and accumulation of authentic plant glycoproteins (antibodies) in plants
- Genome editing in plants (Crispr, RNAi)
- Protein expression in plant cell cultures
The influence of abiotic stress on virus infection in plants
- The effects of "priming" by abiotic stressors (heat, drought, cold) on the course and mechanisms of virus infection