Principal research fields at the Institute of Experimental Botany of the Czech Academy of Sciences are plant genetics, physiology and biotechnology.

In the field of genetics, the institute works on structural and functional genomics, induced mutagenesis and DNA repair or pollen molecular genetics.

Physiological topics include cell biology, hormonal and ecological control of plant growth and development, mechanisms of action of growth regulators, physiology of plant viruses and plant pathophysiology.