Instructions | ÚEB AV ČR

IFIEB provides an open access to our instrumentation and expertise both to internal and external users.
Facility instrumentation is self-operated after introductory training. Unless specifically trained by the IFIEB staff, users are not allowed to operate any equipment. Therefore first, you have to get in contact with the IFIEB stuff to get an introductory training. ifieb@ueb.cas.cz or malinska@ueb.cas.cz
After appropriate trainings and understanding fundaments (e.g. theoretical basics on confocal microscopy - https://youtu.be/t6s9e6IofGE) you will get access to the online booking system and to the microscopes.
IFIEB staff is here for you, so do not hesitate to ask for assistance.
IFIEB provides new users training, assisted equipment sessions, assistance on optimal experimental design, sample handling and data acquisition. On request we help with data analysis.

Book the microscopes via our reservation system.
The signee is responsible for proper handling of the system during the microscope session.
Do not reserve the systems for more than 4 hours a day (between 9 am and 6 pm).
Long-term time-lapse experiments should be scheduled between 6 pm and 9 am the next morning or weekends.
Idle (unused) reservations need to be deleted through the booking system prior to the actual microscopy session.

Access is restricted to registered users ONLY! Non-registered users can accompany registered users on their responsibility. Non-registered users are not permitted to work at the microscope systems in absence of a registered user.
Write each your microscopy session to the reservation book by the microscope.
Report any damages immediately to the imaging facility; mark any abnormalities in the reservation book by the microscope.
Be nice to our microscopes, namely to the objectives!
Leave the workspace clean.
Do not put oil on the air and water objectives, do not overapply oil.
Do not use border/peripheral positions of slides/chambers, there is very high risk of objective scratching.
When cleaning off the objectives, use only lens paper supplied by the imaging facility. To remove immersion oil and clean oil objectives use Isopropanol supplied. Never use technical ethanol in wash bottle for objectives.
Power up the microscope only in the order demonstrated by the imaging facility. Power down with everything in the reverse order. Never run burners, lasers and cameras without their appropriate cooling devices.
Let Argon lasers in standby mode if somebody is scheduled after you. Check if he started to work.
Turn off the system after your work when you are the last user for the day.

Preferentially save your data on your IEB P: drive (\\ds.asuch.cas.cz\home) (capacity 200GB) or on Imaging Facility disc station (\\ds-ueb-if.asuch.cas.cz\home). These data are backed up. Detail instructions how to map these disc stations are by each microscope.
Alternatively save your data temporarily in the system’s DATA hard drive space.

Name your folder with your Surname (not First name only, please). These data are NOT backed up. Please do not store your data simultaneously on DATA hard drive and IF/IEB Disc Station. Clean up your files from DATA hard drive as soon as possible.
Never save your files on the computer’s desktop or C: drive. These data could be deleted.

Preferentially save your data on Imaging Facility disc station (capacity not limited) or on your IEB P: drive (capacity 100GB). Instruction how to map these disc stations are by each microscope and in section D of this web page.
Or Save your data temporarily in the system’s DATA hard drive space. Name your folder with your Surname (not First name only, please).
Never save your files on the computer’s desktop or C: drive. Clean up your files from the system’s hard drive as soon as possible.

Prices in CZK per hour without VAT.

Microscopy system	CzBI access	Academic access
Mirava Polyscope 3D STED, FLIM, MATRIX, adaptive optics	400	800
Zeiss LSM 880 with Airyscan inverted confocal microscope in standard or vertical stage setup Zeiss LSM900 with Airyscan2 Multiplex mode	240 260	480 520
Nikon Spinning Disc inverted spinning disc confocal microscope	220	440
Zeiss Axiovert 200 inverted confocal microscope Zeiss Axio Imager s ApoTome2 Upright wide field fluorescence microscope	80 100	160 200
Stereomicroscope Leica M205FA fluorescence stereomicroscope	80	160
InSituPro VSi automated pipetting station	40	80
RAPID chamber	20	40
Microscopy Assistance	300	500
Image analysis	300	500

Reduced prices within CzechBioImaging access are possible via registration form access or by contacting IFIEB facility ifieb@ueb.cas.cz and must be followed by proper CzBI acknowledgement.